Novel E. coli mutants deficient in blosynthesis of 5-methylamlnomethyl-2-thiouridine

Novel E. coli mutants deficient in biosynthesis of 5-methylaminomethyl-2- thiouridine were isolated based on a phenotype of reduced readthrough at UAG codons. They define 2 new loci trmE and trmF, near 83′ on the E. coli map. These mutants are different from strains carrying trmC mutations, which ar...

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Bibliographic Details
Published in:Nucleic acids research 1984-04, Vol.12 (8), p.3521-3534
Main Authors: Elseviers, Drik, Petrullo, Lynn A., Gallagher, Patricia J.
Format: Article
Language:English
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Summary:Novel E. coli mutants deficient in biosynthesis of 5-methylaminomethyl-2- thiouridine were isolated based on a phenotype of reduced readthrough at UAG codons. They define 2 new loci trmE and trmF, near 83′ on the E. coli map. These mutants are different from strains carrying trmC mutations, which are known to confer a methylation deficiency in biosynthesis of 5-methylaminomethyl-2-thiouridine. tRNA from mutants carrying trmE or trmF mutations was shown to carry 2-thiouridine instead of 5-methylaminomethyl-2-thiouridine. This deficiency affects the triplet binding properties of the mutant tRNA. Our results suggest that the 5-methylaminomethyl group stabilizes the basepairing this modified nucleotide with G, most likely through direct interaction with the ribosomal binding site(s).
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/12.8.3521