The mRNA for a proteinase inhibitor related to the HI-30 domain of inter-α-trypsin inhibitor also encodes α-1-microgiobulin (protein HC

Inter-a-trypsin inhibitor (ITI) is a 180 kd serine proteinase inhibitor found in human serum. Treatment of 180 kd ITI with trypsin releases a 30 kd fragment (HI-30) which contains the anti-proteolytic activity of the high molecular weight form. We have isolated a cDNA clone from a human liver librar...

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Bibliographic Details
Published in:Nucleic acids research 1986-10, Vol.14 (20), p.7839-7850
Main Authors: Kaumeyer, John F., Polazzi, Joseph O., Kotick, Michael P.
Format: Article
Language:English
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Summary:Inter-a-trypsin inhibitor (ITI) is a 180 kd serine proteinase inhibitor found in human serum. Treatment of 180 kd ITI with trypsin releases a 30 kd fragment (HI-30) which contains the anti-proteolytic activity of the high molecular weight form. We have isolated a cDNA clone from a human liver library which codes for HI-30, and have determined its DNA sequence. The mRNA not only codes for HI-30 but also another serum protein, α1-microglobulin, which has not been previously associated with ITI or HI-30. The α-1-microglobulin sequence is found in the amino-terminus of the protein and is preceded by a signal sequence. HI-30 is found at the carboxy-terminus. The two protein sequences are separated by two arginine residues.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/14.20.7839