Formation and stability of alternate-stranded triplex formed with alpha,beta-chimeric oligoDNA

Chimeric oligoDNAs composed of α-anomeric oligonucleotide and β-anomeric oligonucleotide with modified nucleobases and/or phosphodiester backbone are synthesized and their ability to form alternate-stranded triple helix with dsDNA was examined. Substitution of normal phosphodiester linkages with pho...

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Bibliographic Details
Published in:Nucleic Acids Symposium Series 2002-11, Vol.2 (1), p.263-264
Main Authors: Hirose, Yoshihiko, Kadoya, Aimi, Matsumoto, Noritake, Miyashita, Takanori, Moriguchi, Tomohisa, Shinozuka, Kazuo
Format: Article
Language:English
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Summary:Chimeric oligoDNAs composed of α-anomeric oligonucleotide and β-anomeric oligonucleotide with modified nucleobases and/or phosphodiester backbone are synthesized and their ability to form alternate-stranded triple helix with dsDNA was examined. Substitution of normal phosphodiester linkages with phosphorothioate linkages resulted in the prominent destabilization of the alternate-stranded triplex. Meanwhile, the introduction of cationic molecule or intercalative molecule at certain positions of the modified DNA was effective to increase the stability of the triplexes.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/2.1.263