Directed evolution of xylanase J from alkaliphilic Bacillus sp. strain 41M-1: restore of alkaliphily of a mutant with an acidic pH optimum

Alkaliphilic Bacillus sp. strain 41M-1 produces an alkaliphilic xylanase (xylanase J). The newly constructed mutant E177QΔJC had an acidic pH optimum and showed almost no activity at pH 8.0. The alkaliphily of the enzyme was restored by directed evolution. The evolved mutants, Y176S/E177QΔJC and G32...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic Acids Symposium Series 2003-09, Vol.3 (1), p.315-316
Main Authors: Inami, Mayuko, Morokuma, Chihiro, Sugio, Akiko, Tamanoi, Hidenori, Yatsunami, Rie, Nakamura, Satoshi
Format: Article
Language:English
Citations: Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Alkaliphilic Bacillus sp. strain 41M-1 produces an alkaliphilic xylanase (xylanase J). The newly constructed mutant E177QΔJC had an acidic pH optimum and showed almost no activity at pH 8.0. The alkaliphily of the enzyme was restored by directed evolution. The evolved mutants, Y176S/E177QΔJC and G32V/Y176D/E177QΔJC, retained about 30% and 43% activity of their maximal activities and 43% activity of their maximal activities at pH 6.0, respectively.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/3.1.315