Synthesis of double-headed 2-5A-antisense chimeras and their ability to activate human RNase L

The synthesis of a novel 2-5A-antisense chimera having two molecules of 2-5A tetramer at the 5′-terminus of the antisense moiety with an ethylene glycol linker is described. The ability of the synthesized 2-5A antisense chimeras to activate RNase L was estimated by monitoring the cleavage of a targe...

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Bibliographic Details
Published in:Nucleic Acids Symposium Series 2003-09, Vol.3 (1), p.63-64
Main Authors: Ueno, Yoshihito, Okatani, Shusaku, Yamada, Yuuki, Kitade, Yukio
Format: Article
Language:English
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Summary:The synthesis of a novel 2-5A-antisense chimera having two molecules of 2-5A tetramer at the 5′-terminus of the antisense moiety with an ethylene glycol linker is described. The ability of the synthesized 2-5A antisense chimeras to activate RNase L was estimated by monitoring the cleavage of a target RNA by the activated RNase L. It was found that the double-headed 2-5A-antisense chimera linked with two molecules of a butanediol linker more efficiently cleaved the target RNA as compared with the single-headed 2-5A-antisense chimera and the double-headed 2-5A-antisense chimera linked with a molecule of the butanediol linker.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/3.1.63