Antisense oligodeoxynucleotides: useful tool for search and assessment of new targets for anti-malarial drugs

We investigated about targeting for new antimalarial drugs using antisense (AS) oligodeoxynucleotides (ODNs). Synthetic nuclease-resistant ODNs (phosphorothioate (PS) ODNs and ODNs containing 4′α-C-(2-aminoethyl)thymidines (4′-amino ODNs)) which target mitochondrial succinate dehydrogenase (SDH) iro...

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Bibliographic Details
Published in:Nucleic Acids Symposium Series 1999-11, Vol.42 (1), p.89-90
Main Authors: Ikemoto, Naomi, Kim, Hye-Sook, Kanazaki, Makiko, Ueno, Yoshihito, Shuto, Satoshi, Matsuda, Akira, Wataya, Yusuke
Format: Article
Language:English
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Summary:We investigated about targeting for new antimalarial drugs using antisense (AS) oligodeoxynucleotides (ODNs). Synthetic nuclease-resistant ODNs (phosphorothioate (PS) ODNs and ODNs containing 4′α-C-(2-aminoethyl)thymidines (4′-amino ODNs)) which target mitochondrial succinate dehydrogenase (SDH) iron-sulfur subunit (IP), had antimalarial activity (EC50; about 1.0 μM). Furthermore we showed that intra-parasitic SDH IP mRNA levels, which were detected using quantitative RT-PCR assay, were decreased 13% of control after the 24h expose to SDH IP AS. From the results, we conclude that SDH has potential as the target for novel antimalarials, and AS ODNs is effective for search and assessment of targets for new antimalarial drugs.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/42.1.89