Quantification analysis of translation initiation signal in vertebrate mRNAs: Effect of nucleotides at positions +4 ∼ +6 upon efficiency of translation initiation

Concerning initiation the translation signal in vertebrate mRNAs, a consensus sequence, (GCC)GCC(A or G)CCATGG, has been but actual initiation differ from it in a greater or lesser degree. Kozak monitored selection by ribosomes of the first versus second ATG codons as a function of mutations introdu...

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Bibliographic Details
Published in:Nucleic Acids Symposium Series 2000-10, Vol.44 (1), p.77-78
Main Authors: Iida, Yôichi, Kanagu, Daisuke
Format: Article
Language:English
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Summary:Concerning initiation the translation signal in vertebrate mRNAs, a consensus sequence, (GCC)GCC(A or G)CCATGG, has been but actual initiation differ from it in a greater or lesser degree. Kozak monitored selection by ribosomes of the first versus second ATG codons as a function of mutations introduced at positions +4, +5, and +6 of the first ATG codon. Codons possessing G at +4 strongly enhanced selection of the first ATG codon. However, ATG codon recognition was unaffected by most mutations in positions +5 and +6. These data were well understood by our quantification analysis.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/44.1.77