Mutagenesis by damaged deoxyribonucleotides and its prevention by MutT-type hydrolyzing enzymes

Oxidized deoxyribonucleotides, 2-hydroxydeoxyadenosine 5′-triphosphaie (2-OH-dATP) and 8-hydroxydeoxyguanosine 5′-triphosphate (8-OH-dGTP), were introduced into Escherichia coli strains deficient in DNA polymerase IV (a Y-family DNA polymerase encoded in the dinB gene), and the MutT and Orfl35 prote...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic Acids Symposium Series 2004-11, Vol.48 (1), p.271-272
Main Authors: Kamiya, Hiroyuki, Satou, Kazuya, Hori, Mika, Iida, Emiko, Ishiguro, Chieko, Harashima, Hideyoshi
Format: Article
Language:English
Citations: Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Oxidized deoxyribonucleotides, 2-hydroxydeoxyadenosine 5′-triphosphaie (2-OH-dATP) and 8-hydroxydeoxyguanosine 5′-triphosphate (8-OH-dGTP), were introduced into Escherichia coli strains deficient in DNA polymerase IV (a Y-family DNA polymerase encoded in the dinB gene), and the MutT and Orfl35 proteins to examine their in vivo roles in mutagenesis elicited by 2-OH-dATP and 8-OH-dGTP. 2-OH-dATP elicited mutations less efficiently in the dinB− strain than in the wild type strain, suggesting involvement of DNA polymerase IV in 2-OH-dATP-induced mutations. 8-OH-dGTP and 2-OH-dATP elicited mutations more efficiently in mutT− and orfl35− strains, respectively, than those in their isogenic mutT+ and orfl35+ strains. These results indicate that these proteins play important roles in mutagenesis induced by 2-OH-dATP and 8-OH-dGTP in vivo.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/48.1.271