Design of extremely facile 3′- and 5′- ends free molecular beacons using C8 alkylamino substituted 2′-deoxyguanosine

Abstract A novel fluorescent oligonucleotide probe containing pyrene-labeled C8 alkylamino-substituted 2′-deoxyguanosine and a practically useful 3′- and 5′- ends-free self-quenched molecular beacons (MB) were designed. A unique MB detectable by pyrene excimer fluorescence was also demonstrated....

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Bibliographic Details
Published in:Nucleic Acids Symposium Series 2009-09, Vol.53 (1), p.141-142
Main Authors: Matsumoto, Katsuhiko, Shinohara, Yuta, Numajiri, Kyoko, Ishioroshi, Shinya, Morii, Takashi, Saito, Yoshio, Saito, Isao
Format: Article
Language:English
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Summary:Abstract A novel fluorescent oligonucleotide probe containing pyrene-labeled C8 alkylamino-substituted 2′-deoxyguanosine and a practically useful 3′- and 5′- ends-free self-quenched molecular beacons (MB) were designed. A unique MB detectable by pyrene excimer fluorescence was also demonstrated.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/nrp071