Lymphocyte subsets in dialyser eluates : a new parameter of bioincompatibility ?

• Published in: Nephrology, dialysis, transplantation dialysis, transplantation, 1996-06, Vol.11 (6), p.1073-1078
• Main Authors: GROOTEMAN, M. P. C, NUBE, M. J, VAN LIMBEEK, J, SCHOORL, M, VAN HOUTE, A. J
• Format: Article
• Language: English
• Subjects: Adult; Aged; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biocompatible Materials; Biological and medical sciences; Blood Cell Count; Blood Cells - pathology; Cellulose - adverse effects; Cellulose - analogs & derivatives; Emergency and intensive care: renal failure. Dialysis management; Female; Humans; Intensive care medicine; Leukocyte Count; Lymphocyte Activation; Lymphocyte Subsets - pathology; Male; Medical sciences; Membranes, Artificial; Middle Aged; Polymers - adverse effects; Renal Dialysis - adverse effects; Sulfones - adverse effects; Therapeutic Irrigation
• ISSN: 0931-0509; 1460-2385
• DOI: 10.1093/ndt/11.6.1073

During haemodialysis (HD), several adverse reactions in peripheral blood can occur, which have been attributed to the bioincompatibility of the dialyser membrane. Utilizing a dialyser elution technique, we have demonstrated that polymorphonuclear cells (PMN) manifested non-membrane dependent signs of activation during HD with cellulose triacetate (CTA), cuprammonium (CU) and polysulphone (PS) membranes. In the present study, we employed this elution technique to investigate the influence of HD with these membranes on lymphocytes. Eight patients were studied during HD with CTA, CU, and PS dialysers in a randomized crossover design. Dialyser elution was performed after 3 h of HD. Besides total leukocyte count and differentiation, lymphocyte subpopulations and activation status in peripheral blood and dialyser eluates were analysed by flow cytometry. Only with CU was a significant leukocyte decrease observed in peripheral blood at 30 min (P