Dynamics of secretion and metabolism of PTH during hypo‐ and hypercalcaemia in the dog as determined by the ‘intact’ and ‘whole’ PTH assays

Background. Recent evidence has shown that the assay for ‘intact’ parathyroid hormone (I‐PTH) not only reacts with 1–84 PTH but also with large non‐1–84 PTH fragments, most of which is probably 7–84 PTH. As a result, an assay specific for 1–84 PTH named ‘whole’ PTH (W‐PTH) has been developed. The pr...

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Bibliographic Details
Published in:Nephrology, dialysis, transplantation dialysis, transplantation, 2003-06, Vol.18 (6), p.1101-1107
Main Authors: Estepa, Jose C., Lopez, Ignacio, Felsenfeld, Arnold J., Gao, Ping, Cantor, Tom, Rodríguez, Mariano, Aguilera‐Tejero, Escolastico
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
calcium
Calcium Chloride
Disease Models, Animal
Dogs
Edetic Acid
Female
hypercalcaemia
Hypercalcemia - chemically induced
Hypercalcemia - metabolism
hypocalcaemia
Hypocalcemia - chemically induced
Hypocalcemia - metabolism
Immunoradiometric Assay
Infusions, Intravenous
intact PTH
Kidney - physiology
Male
Medical sciences
Metabolic diseases
Miscellaneous
Other metabolic disorders
parathyroid hormone
Parathyroid Hormone - metabolism
Peptide Fragments - blood
whole PTH
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Summary:Background. Recent evidence has shown that the assay for ‘intact’ parathyroid hormone (I‐PTH) not only reacts with 1–84 PTH but also with large non‐1–84 PTH fragments, most of which is probably 7–84 PTH. As a result, an assay specific for 1–84 PTH named ‘whole’ PTH (W‐PTH) has been developed. The present study was designed: (i) to determine whether the W‐PTH assay reliably measures PTH values in the dog; (ii) to evaluate differences between the W‐PTH and I‐PTH assays during hypo‐ and hypercalcaemia; and (iii) to assess the peripheral metabolism of W‐PTH and I‐PTH. Methods. In normal dogs, hypocalcaemia was induced by EDTA infusion and was followed with a 90 min hypocalcaemic clamp. Hypercalcaemia was induced with a calcium infusion. Results. I‐PTH and W‐PTH values increased from 36±8 and 13±3 pg/ml (P=0.01) at baseline to a maximum of 158±40 and 62±15 pg/ml (P=0.02 vs I‐PTH) during hypocalcaemia. The W‐PTH/I‐PTH ratio, 38±4% at baseline, did not change during the induction of hypocalcaemia, but sustained hypocalcaemia increased (P
ISSN:0931-0509
1460-2385
DOI:10.1093/ndt/gfg104