Experimentally Synthesized Plant Chimeras I. In vitro Recovery of Nicotiana tabacum L. Chimeras from Mixed Callus Cultures

Experiments were conducted to develop techniques for synthesizing chimeras between plants of known genotype by utilizing in vitro techniques Chimeral calli composed of green and albino tobacco cells were obtained by initiating callus tissue from mixtures of albino and green cotyledons, hypocotyls, c...

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Bibliographic Details
Published in:Annals of botany 1984-10, Vol.54 (4), p.503-511
Main Authors: MARCOTRIGIANO, M., GOUIN, F R
Format: Article
Language:English
Subjects:
Adventitious shoots
Apical meristems
Callus
callus cultures
Cell lines
cell suspensions
Chemical suspensions
Chimeras
Cultured cells
Genotypes
Plant tissues
Plants
shoot apical meristems
somatic-crossing over
tissue culture
Tobacco
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Summary:Experiments were conducted to develop techniques for synthesizing chimeras between plants of known genotype by utilizing in vitro techniques Chimeral calli composed of green and albino tobacco cells were obtained by initiating callus tissue from mixtures of albino and green cotyledons, hypocotyls, callus cultures and cell suspensions The most effective mixing of genotypes occurred when callus was derived from mixed filtered cell suspensions Upon shoot regeneration, chimeral calli yielded 1317 non-chimeral and four chimeral plants Chimeras may have arisen as a result of experimental procedures or possibly from spontaneous chromosomal abnormalities since leaves of some albino control plants occasionally produced small green islands of cells Explanations for the recovery of a high percentage of non-chimeral shoots are presented
ISSN:0305-7364
1095-8290
DOI:10.1093/oxfordjournals.aob.a086820