Generation of seakale (Crambe maritima L.) plantlets by tissue culture

When placed in shaken liquid culture in MS medium, callus derived from petioles of seakale (Crambe maritima L.) failed to fragment to produce a cell suspension culture. In the absence of 2,4-D, growth was very good, and hollow, dark green, trumpet-shaped bodies were produced, but in the presence of...

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Bibliographic Details
Published in:Annals of botany 1986-08, Vol.58 (2), p.179-181
Main Authors: Drew, R.L.K, Fellows, J.R
Format: Article
Language:English
Subjects:
2,4-D
Agronomy. Soil science and plant productions
Biological and medical sciences
Body tissues
Bridge engineering
Callus
callus culture
Crambe maritima
Crambe maritima L
Cultured cells
Developmental biology
Economic plant physiology
Fundamental and applied biological sciences. Psychology
In vitro culture
in vitro propagation
Petioles
plant development
Plant physiology and development
Plant propagation
Plant roots
Plantlets
Seakale
tissue culture
Tissue culture techniques
Vegetative and sexual reproduction, floral biology, fructification
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Summary:When placed in shaken liquid culture in MS medium, callus derived from petioles of seakale (Crambe maritima L.) failed to fragment to produce a cell suspension culture. In the absence of 2,4-D, growth was very good, and hollow, dark green, trumpet-shaped bodies were produced, but in the presence of 2,4-D, solid, paler green pear-shaped bodies were formed. When grown on filter paper bridges with White's medium, a small proportion of both types of bodies underwent slow, partly-controlled development culminating, after several months, in the formation of plantlets.
ISSN:0305-7364
1095-8290
DOI:10.1093/oxfordjournals.aob.a087195