Influence of polymorphonuclear granulocytes on the zona-free hamster oocyte assay

To gain more insight into a possible detrimental influence of polymorphonuclear granulocytes (PMN) on fertility, PMN were added to spermatozoa and oocytes in the hamster oocyte assay. Addition of 2.5×106/ml PMN to the sperm suspension at the start of the first incubation period (capacitation period)...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1991-09, Vol.6 (8), p.1104-1107
Main Authors: Vogelpoel, F.R., van Kooij, R.J., te Velde, E.R., Verhoef, J.
Format: Article
Language:English
Subjects:
Adult
Animals
Biological and medical sciences
Birth control
Cricetinae
Female
Gynecology. Andrology. Obstetrics
human sperniatozoa
Humans
Male
Medical sciences
Neutrophils - physiology
Oocytes - physiology
oxygen radicals
polymorphonuclear granulocytes
Sperm Capacitation
Sperm-Ovum Interactions - drug effects
Sperm-Ovum Interactions - physiology
Spermatozoa - physiology
Sterility. Assisted procreation
Tetradecanoylphorbol Acetate - pharmacology
Thiourea - pharmacology
Zona Pellucida - physiology
zona-free hamster oocyte assay
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Summary:To gain more insight into a possible detrimental influence of polymorphonuclear granulocytes (PMN) on fertility, PMN were added to spermatozoa and oocytes in the hamster oocyte assay. Addition of 2.5×106/ml PMN to the sperm suspension at the start of the first incubation period (capacitation period) resulted in a significant decrease (P
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a137493