Sealed mini-chamber of variable depth for direct observation and extended evaluation of sperm motility under the influence of various gases

A new chamber for microscopical observation of living cells, e.g. spermatozoa, under hermetically sealed conditions and over an extended period is described. Motile spermatozoa were serially observed for several hours and the effect of various gases on sperm motility has been studied. The chamber co...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1991-10, Vol.6 (9), p.1275-1278
Main Author: MAKLER, A
Format: Article
Language:English
Subjects:
Atmosphere Exposure Chambers
Biological and medical sciences
chamber for sperm analysis
Evaluation Studies as Topic
extended sperm analysis
Gases - pharmacology
gases and sperm motility
Genital system. Mammary gland
Humans
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Sperm Motility
sperm survival
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Summary:A new chamber for microscopical observation of living cells, e.g. spermatozoa, under hermetically sealed conditions and over an extended period is described. Motile spermatozoa were serially observed for several hours and the effect of various gases on sperm motility has been studied. The chamber could also be used to study the effect of various toxic gases in the fields of microbiology and toxicology.
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a137525