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The Major Acidic Glycolipids from the Kidney of the Pacific Salmon (Oncorhynchus keta): Characterization of a Novel Ganglioside, Fucosy 1-N-acety lgalactosaminy 1- GM1
Two fractions of a major ganglioside from the kidney of the pacific salmon, Oncorhynchus keta, were eluted from a DEAE-Sephadex column in the monosialosyl fraction. The faster moving ganglioside (XI) on TLC was separated from the slower moving one (X2) by HPLC using a silica beads column. By methyla...
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Published in: | Journal of biochemistry (Tokyo) 1999-11, Vol.126 (5), p.962-968 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Two fractions of a major ganglioside from the kidney of the pacific salmon, Oncorhynchus keta, were eluted from a DEAE-Sephadex column in the monosialosyl fraction. The faster moving ganglioside (XI) on TLC was separated from the slower moving one (X2) by HPLC using a silica beads column. By methylation analysis, chemical and enzymatic degradation, reaction with monoclonal antibodies, LSIMS, and ‘H-NMR spectroscopy, XI was determined to be a monosialosyl ganglioside belonging to the ganglio-series with a unique Fuc |
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ISSN: | 0021-924X |
DOI: | 10.1093/oxfordjournals.jbchem.a022541 |