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The Major Acidic Glycolipids from the Kidney of the Pacific Salmon (Oncorhynchus keta): Characterization of a Novel Ganglioside, Fucosy 1-N-acety lgalactosaminy 1- GM1

Two fractions of a major ganglioside from the kidney of the pacific salmon, Oncorhynchus keta, were eluted from a DEAE-Sephadex column in the monosialosyl fraction. The faster moving ganglioside (XI) on TLC was separated from the slower moving one (X2) by HPLC using a silica beads column. By methyla...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1999-11, Vol.126 (5), p.962-968
Main Authors: Niimura, Yukio, Tomori, Michiko, Tadano-Aritomi, Keiko, Toida, Toshihiko, Ishizuka, Ineo
Format: Article
Language:English
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Summary:Two fractions of a major ganglioside from the kidney of the pacific salmon, Oncorhynchus keta, were eluted from a DEAE-Sephadex column in the monosialosyl fraction. The faster moving ganglioside (XI) on TLC was separated from the slower moving one (X2) by HPLC using a silica beads column. By methylation analysis, chemical and enzymatic degradation, reaction with monoclonal antibodies, LSIMS, and ‘H-NMR spectroscopy, XI was determined to be a monosialosyl ganglioside belonging to the ganglio-series with a unique Fuc
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a022541