Trans-Kingdom Conjugation Between Agrobacterium tumfaciens and Saccharomyces cerevisiae, a Bacterium and a Yeast

For conjugation between prokaryotic Agrobacterium tumefaciens and eukaryotic Saccharomyces cerevisiae, we constructed two novel conjugative plasmids. A. tumefaciens transmitted the plasmids to S. cerevisiae with the aid of tra genes on a helper plasmid. The transmitted plasmids retained their origin...

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Bibliographic Details
Published in:Plant and cell physiology 1996-01, Vol.37 (1), p.103-106
Main Authors: Sawasaki, Yukiko, Inomata, Koji, Yoshida, Kazuo
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Conjugative plasmids
Saccharomyces cerevisiae
Ti plasmid
Trans-kingdom conjugation
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Summary:For conjugation between prokaryotic Agrobacterium tumefaciens and eukaryotic Saccharomyces cerevisiae, we constructed two novel conjugative plasmids. A. tumefaciens transmitted the plasmids to S. cerevisiae with the aid of tra genes on a helper plasmid. The transmitted plasmids retained their original structure and function in transconjugant yeasts. The presence of Ti plasmid barely affected the trans-kingdom conjugation.
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a028906