Expression of bacterial chorismate pyruvate-lyase in tobacco [Nicotiana tabacum]: Evidence for the presence of chorismate in the plant cytosol

The bacterial gene ubiC encodes chorismate pyruvate-lyase, an enzyme which converts chorismate to 4-hydroxybenzoate and which is not normally present in plants. The UbiC protein was expressed in tobacco, with targeting of the gene product either to the plastids or to the cytosol. In both cases, chor...

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Bibliographic Details
Published in:Plant and cell physiology 1998-11, Vol.39 (11), p.1240-1244
Main Authors: Sommer, S. (Universitaet Tuebingen (Germany)), Heide, L
Format: Article
Language:English
Subjects:
4-Hydroxybenzoic acid
Agrobacterium tumefaciens
Agrobacterium tumefaciens - enzymology
Agrobacterium tumefaciens - genetics
Agronomy. Soil science and plant productions
Base Sequence
biochemical pathways
Biological and medical sciences
Chorismate
CITOPLASMA
Consensus Sequence
CYTOPLASM
CYTOPLASME
cytosol
Economic plant physiology
enzyme activity
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
gene expression
gene transfer
genes
genetic regulation
LIASAS
localization
LYASE
LYASES
Metabolism
Metabolism. Physicochemical requirements
metabolites
Nicotiana
NICOTIANA TABACUM
Nitrogen metabolism and other ones (excepting carbon metabolism)
NUCLEOTIDE SEQUENCE
Nutrition. Photosynthesis. Respiration. Metabolism
Oxo-Acid-Lyases - biosynthesis
Oxo-Acid-Lyases - genetics
phenolic acids
PIRUVATOS
Plant physiology and development
Plants, Genetically Modified
Plants, Toxic
plastids
PYRUVATE
PYRUVATES
pyruvic acid
Recombinant Proteins - biosynthesis
SECUENCIA NUCLEOTIDICA
Sequence Alignment
SEQUENCE NUCLEOTIDIQUE
shikimic acid
ubiC
ubic genes
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Description
Summary:The bacterial gene ubiC encodes chorismate pyruvate-lyase, an enzyme which converts chorismate to 4-hydroxybenzoate and which is not normally present in plants. The UbiC protein was expressed in tobacco, with targeting of the gene product either to the plastids or to the cytosol. In both cases, chorismate pyruvate-lyase activity and a resulting formation of 4-hydroxybenzoate was detected. This suggests that chorismate, a metabolite of the shikimate pathway, is present not only in the plastids but also in the cytosol of plant cells.
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a029326