Salicylic acid induces extracellular superoxide generation followed by an increase in cytosolic calcium ion in tobacco [Nicotiana tabacum] suspension culture: The earliest events in salicylic acid signal transduction

Addition of salicylic acid (SA) to tobacco (Nicotiana tabacum) suspension culture immediately induced a rapid and transient generation of superoxide anion (O2(-)), followed by a transient increase in cytosolic free calcium ion concentration ([Ca(2+)]c). The level of SA-induced O2(-) was lowered by t...

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Bibliographic Details
Published in:Plant and cell physiology 1998-07, Vol.39 (7), p.721-730
Main Authors: Kawano, T. (Nagoya Univ. (Japan)), Sahashi, N, Takahashi, K, Uozumi, N, Muto, S
Format: Article
Language:English
Subjects:
ACIDE SALICYLIQUE
ACIDO SALICILICO
Biological and medical sciences
CALCIO
CALCIUM
Cell physiology
CULTURE TECHNIQUES
CYTOPLASMIC ORGANELLES
Cytosolic calcium
Extracellular peroxidase
FORECASTING
Fundamental and applied biological sciences. Psychology
IONISATION
IONIZACION
IONIZATION
Molecular and cellular biology
NICOTIANA TABACUM
ORGANITE CELLULAIRE
ORGANULOS CITOPLASMICOS
PEROXIDES
PEROXIDOS
PEROXYDE
PRODUCCION
PRODUCTION
Salicylic acid
SALICYLIC ACIDS
Signal transduction
Superoxide
TECHNIQUE DE CULTURE
TECHNIQUE DE PREVISION
TECNICAS DE CULTIVO
TECNICAS DE PREDICCION
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Summary:Addition of salicylic acid (SA) to tobacco (Nicotiana tabacum) suspension culture immediately induced a rapid and transient generation of superoxide anion (O2(-)), followed by a transient increase in cytosolic free calcium ion concentration ([Ca(2+)]c). The level of SA-induced O2(-) was lowered by treatment with several scavengers of active oxygen species and a peroxidase inhibitor, but not with an NADPH oxidase inhibitor. The Sa-induced [Ca(2+)]c, elevation was also lowered by inhibitors which effectively lowered the O2(-) level. Inhibition of [Ca(2+)]c elevation by Ca(2+) channel blockers and a Ca(2+) chelator indicated that extracellular Ca(2+) was responsible for the increased [Ca(2+)]c. Among the several SA analogs, only compounds that actively induced the O2(-) generation also elevated [Ca(2+)]c. In addition, the inhibitory effects of SA analogs on catalase activity correlated well with their effects on the O2(-) generation and the [Ca(2+)]c elevation. SA-dependent O2(-) generation was shown to occur extracellularly, requiring both H2O2 and at least on e proteinaceous factor excreted from the cells. This factor was determined to be a salicylhydroxamic acid-sensitive extracellular guaiacol-utilizing peroxidase
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a029426