Correlation between induction of auxin-nonrequiring tobacco calluses and increase in inhibitor(s) of IAA-destruction activity

Decreased IAA-destruction activity by tobacco calluses (T22 and XD6S2) was observed only when the calluses were cultured on a medium favorable for auxin-nonrequiring callus induction. The medium used to induce auxin-nonrequiring calluses from auxin-requiring ones contained 0.1 mg/liter IAA for callu...

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Bibliographic Details
Published in:Plant and cell physiology 1979-02, Vol.20 (1), p.29-42
Main Author: Syono K
Format: Article
Language:English
Subjects:
Auxin protector
Habituation
IAA-destruction activity
Tobacco callus
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Summary:Decreased IAA-destruction activity by tobacco calluses (T22 and XD6S2) was observed only when the calluses were cultured on a medium favorable for auxin-nonrequiring callus induction. The medium used to induce auxin-nonrequiring calluses from auxin-requiring ones contained 0.1 mg/liter IAA for callus T22 and 10 mg/liter NAA for XD6S2. It was clearly demonstrated both in vivo and in vitro that the result was not due to decreased IAA-destroying enzyme activity itself, but to the increase of an inhibitor(s) of IAA-destruction activity. The accumulated inhibitor(s), which induced a lag in IAA oxidation, was a heat-stable low molecular weight substance(s), unstable in air at room temperature. The possible involvement of an inhibitor(s) of IAA-destruction in the process of induction for auxin-nonrequiring calluses was discussed.
ISSN:0032-0781
1471-9053
1471-9053
DOI:10.1093/oxfordjournals.pcp.a075803