Biosynthesis of cytokinins by tobacco cell cultures

In synchronously cultured tobacco cells (Nicotiana tabacum cv. Xanthi), the incorporation of U-14C-adenosine into butanol-soluble cytokinins in vivo was studied. The radioactivity was incorporated into zeatin, ribosylzeatin, isopentenyladenosine and glucosylzeatin after 20 min. The radioactive cytok...

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Bibliographic Details
Published in:Plant and cell physiology 1980-11, Vol.21 (8), p.1143-1150
Main Authors: Nishinari, Noriko, Syōno, Kunihiko
Format: Article
Language:English
Subjects:
Biosynthesis
Cell culture
Cytokinins
Nicotiana tabacum
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Summary:In synchronously cultured tobacco cells (Nicotiana tabacum cv. Xanthi), the incorporation of U-14C-adenosine into butanol-soluble cytokinins in vivo was studied. The radioactivity was incorporated into zeatin, ribosylzeatin, isopentenyladenosine and glucosylzeatin after 20 min. The radioactive cytokinins were identified by thin-layer chromatography and high performance liquid chromatography. From the short time course of the incorporation of 14C-adenosine into butanol-soluble cytokinins, the presence of the following biosynthetic pathway in vivo was suggested: adenosine is convered into isopentenyladenosine and then into zeatin via ribosylzeatin. The biosynthetic pathway of free cytokinins in vivo is compared with that in vitro.
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a076113