Oxidation rate of monomeric radish cytochrome f with plastocyanin

The reaction rate of reduced monomeric cytochrome f with oxidized plastocyanin, both purified from Japanese radish, was determined by a stopped-flow method. The oxidation rate constant was 6.0 × 107 M1sec1 at pH 7.0 and 25°C, which is slightly higher than the value reported by Wood [(1974) Biochim....

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Bibliographic Details
Published in:Plant and cell physiology 1981-02, Vol.22 (1), p.33-39
Main Authors: Tanaka, K, Takahashi, M, Asada, K. (Kyoto Univ., Uji (Japan). Research Inst. for Food Science)
Format: Article
Language:English
Subjects:
CHEMICOPHYSICAL PROPERTIES
CROPS
CRUCIFERAE
Cytochrome f oxidation
DICOTYLEDONS
ECONOMIC PLANTS
METALLOPROTEINS
ORGANIC COMPOUNDS
OXIDATION
PIGMENTS
plant biochemistry
plant physiology
PLANTS
Plastocyanin
Polylysine effect
PROTEINS
Radish
RAPHANUS
RAPHANUS SATIVUS
RHOEADALES
ROOT CROPS
ROOT VEGETABLES
VEGETABLE CROPS
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Summary:The reaction rate of reduced monomeric cytochrome f with oxidized plastocyanin, both purified from Japanese radish, was determined by a stopped-flow method. The oxidation rate constant was 6.0 × 107 M1sec1 at pH 7.0 and 25°C, which is slightly higher than the value reported by Wood [(1974) Biochim. Biophys. Acta 357 : 370] for oligomeric parsley cytochrome f Thermodynamic parameters also were determined to be 56 KJ M−1 for activation enthalpy and 90 J M−1 K−1 for activation entropy. Neither a pH from 6 to 9 nor the addition of NaCl, polylysine, histone or polyaspartate affected the rate constant.
ISSN:0032-0781
1471-9053
1471-9053
DOI:10.1093/oxfordjournals.pcp.a076143