Isolation of the red-light-absorbing form of phytochrome from light-grown pea shoots

• Published in: Plant and cell physiology 1981-11, Vol.22 (7), p.1165-1173
• Main Authors: Shimazaki, Y, Moriyasu, Y. (Tokyo Univ. (Japan). Faculty of Science), Pratt, L.H, Furuya, M
• Format: Article
• Language: English
• Subjects: ALIMENTOS; ANALISIS; ANALISIS QUIMICO; ANALYSE; ANALYSE CHIMIQUE; ANALYSIS; ANATOMIA; ANATOMIA DE LA PLANTA; ANATOMIE; ANATOMIE VEGETALE; ANATOMY; CHEMICAL ANALYSIS; CHEMICOPHYSICAL PROPERTIES; CROPS; CULTIVOS; CULTIVOS DE GRANO; DICOTILEDONEAS; DICOTYLEDONE; DICOTYLEDONS; ECONOMIC PLANTS; ESPECTROMETRIA; ESTADO FISICO; ETAT PHYSIQUE; FEED CROPS; FEED LEGUMES; FOODS; FOTOSENSIBILIDAD; GRAIN CROPS; GRAIN LEGUMES; HORTALIZAS (PLANTAS); HORTALIZAS LEGUMINOSAS; Immunochemistry (phytochrome); IMMUNOLOGICAL TECHNIQUES; LEGUMES; LEGUMINEUSE; LEGUMINEUSE A GRAINS; LEGUMINEUSE FOURRAGERE; LEGUMINEUSE LEGUMIERE; LEGUMINOSAE; LEGUMINOSAS; LEGUMINOSAS DE GRANO; LEGUMINOSAS FORRAJERAS; LIGHT; LUMIERE; LUZ; METHODE; METHODS; METODOS; OPTICAL PROPERTIES; PAPILIONOIDEAE; Pea shoot; PHOTOSENSIBILITE; PHOTOSENSITIVITY; PHYSICAL STATES; Phytochrome; PIGMENT; PIGMENTOS; PIGMENTS; PISUM; PISUM SATIVUM; PLANT ANATOMY; PLANTAS; PLANTAS DE VALOR ECONOMICO; PLANTAS FORRAJERAS; PLANTE; PLANTE A GRAINS; PLANTE D'INTERET ECONOMIQUE; PLANTE DE CULTURE; PLANTE FOURRAGERE; PLANTE LEGUMIERE; PLANTS; PRODUIT ALIMENTAIRE; PROPIEDADES FISICO-QUIMICAS; PROPIEDADES OPTICAS; PROPRIETE OPTIQUE; PROPRIETE PHYSICO-CHIMIQUE; RADIACIONES; RADIATION; RADIATIONS; ROSALES; SPECTROMETRIE; SPECTROMETRY; Spectrophotometry (phytochrome); STEMS; TALLO; TECHNIQUE IMMUNOLOGIQUE; TECNICAS INMUNOLOGICAS; TIGE; VEGETABLE CROPS; VEGETABLE LEGUMES
• ISSN: 0032-0781; 1471-9053; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a076267

Phytochrome was extracted from both light-grown and dark-grown shoots of Pisum and partially purified by brushite chromatography and ammonium sulfate fractionation. About 160–270 ng of phytochrome per g green tissue extracted was recovered after the partial purification while about 5.1–8.6 μg of phytochrome per g etiolated tissue was recovered. Only the red-light-absorbing form of phytochrome was detected in extracts prepared from both light- and dark-grown tissue, even though the light-grown tissue was harvested in daylight and purification was done entirely at 0–4°C with only a dim green safe light. No significant differences were found between phytochrome purified from green and etiolated tissues, either in their spectral properties or in their immunochemical reactivity against antietiolated-zucchini-phytochrome serum.