Protein synthesis linked to respiration and phosphorylation in intact Euglena mitochondria

• Published in: Plant and cell physiology 1986-01, Vol.27 (1), p.177-182
• Main Authors: Delorme, E. (Brandeis Univ., Waltham, MA (USA)), Gomez-Silva, B, Stern, A.I, Schiff, J.A
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Euglena gracilis var. bacillaris; FOSFORILACION; Fundamental and applied biological sciences. Psychology; MASTIGOPHORA; Metabolism; MITOCHONDRIA; MITOCHONDRIE; MITOCONDRIA; Oxidative phosphorylation; PHOSPHORYLATION; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; PROTEIN SYNTHESIS; RESPIRACION; RESPIRATION; SINTESIS DE PROTEINAS; SYNTHESE PROTEIQUE
• ISSN: 0032-0781; 1471-9053; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a077079

Highly purified condensed mitochondria obtained from bleached mutant. W10BSmL of Euglena gracilis Klebs var bacillaris Cori incorporate [35S]methionine into protein when fortified with malate, ADP, Mg2+, phosphate and a sucrose osmoticum. Twenty to twenty-five polypeptide bands were found to be labeled in organello when the labeled protein was subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis. Methionine incorporation, but not respiration or oxidative phosphorylation, was blocked by chloramphenicol and other 70S ribosomal translation inhibitors but cycloheximide and ribonuclease were without effect. Inhibitors of electron transport and uncouplers of oxidative phosphorylation were excellent inhibitors of protein synthesis. Thus, these mitochondrial preparations carry out protein synthesis in organello that is linked to respiration and oxidative phosphorylation.