Requirement of manganese for electron donation of hydrogen peroxide in photosystem II reaction center complex

Mn2+ was required for the electron donating reaction from H2O2, but not for that from diphenylcarbazide (DPC), in the PS II reaction center complex which was prepared from spinach chloroplasts by Triton X-100 extraction. The reaction center complex showed a high activity of 2,6-dichloroindophenol (D...

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Bibliographic Details
Published in:Plant and cell physiology 1987-07, Vol.28 (5), p.767-773
Main Authors: Inoue, H. (Toyama Univ. (Japan)), Wada, T
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell structures and functions
Chloroplast, photosynthetic membrane and photosynthesis
FOTOSINTESIS
Fundamental and applied biological sciences. Psychology
HYDROGEN PEROXIDE
MANGANESE
MANGANESO
Molecular and cellular biology
PEROXIDO DE HIDROGENO
PEROXYDE D'HYDROGENE
PHOTOSYNTHESE
PHOTOSYNTHESIS
Photosystem II
Reaction center
Spinach (Spinacia oleracea L.)
SPINACIA OLERACEA
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Summary:Mn2+ was required for the electron donating reaction from H2O2, but not for that from diphenylcarbazide (DPC), in the PS II reaction center complex which was prepared from spinach chloroplasts by Triton X-100 extraction. The reaction center complex showed a high activity of 2,6-dichloroindophenol (DCIP) photoreduction in the presence of DPC, but a low activity with H2O2. The H2O2-supported DCIP photoreduction was suppressed by EDTA and enhanced by a small amount of Mn2+. Ca2+ and Mg2+ could not replace Mn2+. The activation by Mn2+ and its binding showed two binding sites of Mn2+ in the reaction center complex, with high (1.5×107 M−1) and low (1 × 106 M−1) binding constants.
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a077357