Measurement of serine acetyltransferase activity in crude plant extracts by a coupled assay system using cysteine synthase

Serine acetyltransferase (SATase) (EC 2.3.1.30) catalyzes the formation of Oacetyl-L-serine (OAS) from L-serine in the presence of acetyl-CoA. A novel assay method was developed for measuring this enzyme activity in extracts from plant tissues. The assay consists of a coupled system in which the OAS...

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Bibliographic Details
Published in:Plant and cell physiology 1987-07, Vol.28 (5), p.885-891
Main Authors: Nakamura, K. (Chiba Univ., Matsudo (Japan). Faculty of Horticulture), Hayama, A, Masada, M, Fukushima, K, Tamura, G
Format: Article
Language:English
Subjects:
BIOASSAY
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
Cell biochemistry
Cell physiology
CISTEINA
CYSTEINE
Cysteine biosynthesis
ENSAYO BIOLOGICO
ESSAI BIOLOGIQUE
EXTRACTOS
EXTRACTS
EXTRAIT
Fundamental and applied biological sciences. Psychology
New assay system
Plant physiology and development
SERINA
SERINE
Serine acetyltransferase
Sulfate assimilation
TRANSFERASAS
TRANSFERASE
TRANSFERASES
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Summary:Serine acetyltransferase (SATase) (EC 2.3.1.30) catalyzes the formation of Oacetyl-L-serine (OAS) from L-serine in the presence of acetyl-CoA. A novel assay method was developed for measuring this enzyme activity in extracts from plant tissues. The assay consists of a coupled system in which the OAS formed is converted to cysteine by the addition of cysteine synthase (CSase) (EC 4.2.99.8). Cysteine thus formed is determined colorimetrically and serves as a measure for SATase activity. This method is rapid, simple and sensitive, and can be readily adapted for measurement of SATase activity in crude tissue extracts or homogenates.
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a077370