Role of chitinase and chitin oligosaccharides in lignification response of cultured carrot [Daucus carota] cells treated with mycelial walls

Chitinase activity was induced in cultured carrot cells by incubation with mycelial walls of a fungus, Chaetomium globosum. Both intra- and extracellular chitinases were resolved into four components by gel filtration chromatography. The extracellular enzymes liberated soluble oligosaccharides of di...

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Bibliographic Details
Published in:Plant and cell physiology 1988-04, Vol.29 (3), p.527-531
Main Authors: Kurosaki, F. (Toyama Medical and Pharmaceutical Univ. (Japan)), Tashiro, N, Nishi, A
Format: Article
Language:English
Subjects:
ACIDE PHENOLIQUE
ACIDOS FENOLICOS
CELL CULTURE
CELL WALLS
CHITIN
CHITINASE
CHITINE
CULTIVO DE CELULAS
CULTURE DE CELLULES
DAUCUS CAROTA
LIGNIFICACION
LIGNIFICATION
MICELIO
Mycelial walls
MYCELIUM
Oligosaccharides
PARED CELULAR
PAROI CELLULAIRE
PHENOLIC ACIDS
QUITINA
QUITINASA
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Summary:Chitinase activity was induced in cultured carrot cells by incubation with mycelial walls of a fungus, Chaetomium globosum. Both intra- and extracellular chitinases were resolved into four components by gel filtration chromatography. The extracellular enzymes liberated soluble oligosaccharides of different sizes from insoluble chitin, suggesting that these carrot chitinases are endo-hydrolases. The solubilized chitinase digests obtained from insoluble mycelial walls of C. globosum and chitin were fractionated by gel filtration chromatography, and the elicitor activity of each fraction for the accumulation of phenolic acids in cultured carrot cells was determined. In both solubilized fragments of fungal walls and of chitin, elicitor-active oligosaccharides were distributed in many fractions, however, potent activity for inducing phenolic acid synthesis was observed in the high molecular weight fractions.
ISSN:0032-0781
1471-9053
1471-9053
DOI:10.1093/oxfordjournals.pcp.a077525