Establishment of a cell-free system for the biosynthesis of nicotianamine [in Hordeum vulgare]

A cell-free system has been reported for the biosynthesis of nicotianamine, a putative precursor of mugineic acid and its derivatives (MAs). L-[l-14C]methionine was incubated with a crude homogenate of iron-deficient barley roots, and the reaction products were analyzed by HPLC and TLC. Radioactive...

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Bibliographic Details
Published in:Plant and cell physiology 1989-07, Vol.30 (5), p.673-677
Main Authors: Shojima, S. (Tokyo Univ. (Japan). Faculty of Agriculture), Nishizawa, N, Mori, S
Format: Article
Language:English
Subjects:
Barley
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
CELL CULTURE
Cell-free system
CULTIVO DE CELULAS
CULTURE DE CELLULE
DEFICIENCY DISEASES
ENFERMEDADES CARENCIALES
FER
Fundamental and applied biological sciences. Psychology
HIERRO
HORDEUM VULGARE
IRON
Iron deficiency
MALADIE DE CARENCE
Metabolism
Metabolism. Physicochemical requirements
Mugineic acid biosynthesis
Nicotianamine
Phytosiderophores
Plant physiology and development
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Summary:A cell-free system has been reported for the biosynthesis of nicotianamine, a putative precursor of mugineic acid and its derivatives (MAs). L-[l-14C]methionine was incubated with a crude homogenate of iron-deficient barley roots, and the reaction products were analyzed by HPLC and TLC. Radioactive nicotianamine was formed enzymatically in a reaction that was dependent on ATP. The effect of aminooxyacetic acid (AOA) was compared between systems for the synthesis of nicotianamine in vivo and in vitro.
ISSN:0032-0781
1471-9053
1471-9053
0032-0781
DOI:10.1093/oxfordjournals.pcp.a077792