Immunocytochemical localization of uricase in peroxisomes of soybean cotyledons

Antibodies specific for nodule uricase were used for immunocytochemistry to demonstrate the presence of uricase in cotyledons of soybean (Glycine max) during germination and early seedling growth. The enzyme was localized exclusively in peroxisomes.

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Bibliographic Details
Published in:Plant and cell physiology 1994, Vol.35 (6), p.979-982
Main Authors: Damsz, B. (Purdue Univ., West Lafayette (USA)), Dannenhoffer, J.M, Bell, J.A, Webb, M.A
Format: Article
Language:English
Subjects:
COTILEDONES
COTYLEDON
COTYLEDONS
FORMATION DE NODOSITES
Glycine
GLYCINE MAX
Immunolabeling
IMMUNOLOGICAL TECHNIQUES
NODULACION
Nodulin
OXIDOREDUCTASES
OXIDORREDUCTASAS
OXYDOREDUCTASE
ROOT NODULATION
Root nodule
TECHNIQUE IMMUNOLOGIQUE
TECNICAS INMUNOLOGICAS
Uricase (EC 1.7.3.3)
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Description
Summary:Antibodies specific for nodule uricase were used for immunocytochemistry to demonstrate the presence of uricase in cotyledons of soybean (Glycine max) during germination and early seedling growth. The enzyme was localized exclusively in peroxisomes.
ISSN:0032-0781
1471-9053
1471-9053
DOI:10.1093/oxfordjournals.pcp.a078686