Observations on the alpha-amylolysis pattern of some waxy maize starches from inbred line Ia453

Maize starches of the endosperm mutants waxy (wx), dull:waxy (duwx), and amylose-extender:dull:waxy (aeduwx) from inbred line Ia453 lack amylose. However, in addition to high molecular weight (HMW) amylopectin, the duwx and aeduwx starches contained 40 and 80%, respectively, intermediate branched ma...

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Bibliographic Details
Published in:Cereal chemistry 2000-09, Vol.77 (5), p.657-664
Main Authors: Bertoft, E, Boyer, C, Manelius, R, Avall, A.K
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
alpha-amylase
amylopectin
amylose
Biological and medical sciences
corn starch
dextrins
endosperm
Food industries
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
genotype
granules
inbred lines
methanol
molecular weight
mutants
Starch and starchy product industries
Varietal selection. Specialized plant breeding, plant breeding aims
waxy corn
Yield, quality, earliness, varia
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Summary:Maize starches of the endosperm mutants waxy (wx), dull:waxy (duwx), and amylose-extender:dull:waxy (aeduwx) from inbred line Ia453 lack amylose. However, in addition to high molecular weight (HMW) amylopectin, the duwx and aeduwx starches contained 40 and 80%, respectively, intermediate branched material of low molecular weight (LMW). As gelatinized, the amylopectin of the wx starch was easily hydrolyzed into small dextrins by the alpha-amylase of B. amyloliquefaciens, but components of duwx and aeduwx possessed partial resistance to amylolytic attack. Residual material of intermediate size obtained by a 4-hr alpha-amylolysis could not be separated from LMW dextrins by fractional precipitation in methanol. It is suggested that this material possessed a more regularly branched structure, in which the D-glucosyl chain segments were too short to allow alpha-amylase action. The granular starches of duwx and aeduwx genotypes were initially considerably more resistant than the wx sample to alpha-amylase attack. This was possibly due to an altered structure in the amylopectin component or the high content of intermediate material in the former granules.
ISSN:0009-0352
1943-3638
DOI:10.1094/CCHEM.2000.77.5.657