Reactive Oxygen Species Contribute to Symptomless, Extreme Resistance to Potato virus X in Tobacco

• Published in: Phytopathology 2021-10, Vol.111 (10), p.1870-1884
• Main Authors: Király, Lóránt, Albert, Réka, Zsemberi, Orsolya, Schwarczinger, Ildikó, Hafez, Yaser Mohamed, Künstler, András
• Format: Article
• Language: English
• Subjects: Disease Susceptibility; Nicotiana; Plant Diseases; Potexvirus - genetics; Reactive Oxygen Species
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/PHYTO-12-20-0540-R

Here we show that in tobacco ( cultivar Samsun NN ) the development of gene-mediated, symptomless, extreme resistance to (PVX) is preceded by an early, intensive accumulation of the reactive oxygen species (ROS) superoxide (O · ), evident between 1 and 6 h after inoculation and associated with increased nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activities. This suggests a direct contribution of this ROS to virus restriction during symptomless, extreme resistance. Superoxide inhibition in PVX-inoculated leaves by infiltration of antioxidants (superoxide dismutase [SOD] and catalase [CAT]) partially suppresses extreme resistance in parallel with the appearance of localized leaf necrosis resembling a hypersensitive resistance (HR) response. F progeny from crosses of and ferritin overproducer (deficient in production of the ROS OH·) tobaccos also display a suppressed extreme resistance to PVX, because significantly increased virus levels are coupled to HR, suggesting a role of the hydroxyl radical (OH·) in this symptomless antiviral defense. In addition, treatment of PVX-susceptible tobacco with a superoxide-generating agent (riboflavin/methionine) results in HR-like symptoms and reduced PVX titers. Finally, by comparing defense responses during PVX-elicited symptomless, extreme resistance and HR-type resistance elicited by , we conclude that defense reactions typical of an HR (e.g., induction of cell death/ROS-regulator genes and antioxidants) are early and transient in the course of extreme resistance. Our results demonstrate the contribution of early accumulation of ROS (superoxide, OH·) in limiting PVX replication during symptomless extreme resistance and support earlier findings that virus-elicited HR represents a delayed, slower resistance response than symptomless, extreme resistance.