Restriction fragment length polymorphisms in the nuclear ribosomal DNA of four Laccaria spp.: L. bicolor, L. laccata, L. proxima, and L. amethystina

Restriction fragment length Polymorphisms (RFLPs) were examined in the nuclear ribosomal DNA (rDNA) of 29 isolates of Laccaria bicolor, eight of L. laccata, three of L. proxima, and two of L, amethystina. Whole-cell DNAs were digested with BamHI, BglII, EcoRI, HindIII, and PstI and probed with clone...

Full description

Saved in:
Bibliographic Details
Published in:Phytopathology 1990-12, Vol.80 (12), p.1312-1317
Main Authors: Gardes, M. (Universite Laval, Quebec, Que., Canada), Fortin, J.A, Mueller, G.M, Kropp, B.R
Format: Article
Language:English
Subjects:
ADN
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fungi
LACCARIA
MYCORHIZE
MYCORRHIZAE
Plant cytology, morphology, systematics, chorology and evolution
POLIMORFISMO
POLYMORPHISME
RIBOSOMAS
RIBOSOME
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Thallophyta
VARIACION GENETICA
VARIATION GENETIQUE
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Restriction fragment length Polymorphisms (RFLPs) were examined in the nuclear ribosomal DNA (rDNA) of 29 isolates of Laccaria bicolor, eight of L. laccata, three of L. proxima, and two of L, amethystina. Whole-cell DNAs were digested with BamHI, BglII, EcoRI, HindIII, and PstI and probed with cloned rDNA from Armillaria ostoyae by Southern hybridizations. The observed variability suggests that rDNA restriction fragment phenotypes can be used as additional taxonomic characters for comparing Laccaria spp., since the four Laccaria spp., as well as four biological species within L. laccata, were distinguishable. North American isolates of L. bicolor appear to constitute a relatively homogenous group that is distinguishable from European isolates. Large variations in RFLP patterns are detected among isolates of L. laccata, reflecting the morphological, physiological, and genetic heterogeneity of this species. The nuclear ribosomal repeat unit has apparently diverged in L. bicolor and L. amethystina on the two continents and among biological species of L. laccata. Most of the intraspecific polymorphism in rDNA restriction fragment length was observed after digestion with BglII and EcoRI. Mixed rDNA types that differ at least in repeat unit length were also detected with these two enzymes within seven dikaryotic isolates of L. bicolor collected from the field
ISSN:0031-949X
1943-7684
DOI:10.1094/Phyto-80-1312