Preparation and characterization of monoclonal antibodies to double-stranded RNA

Monoclonal antibodies to double-stranded RNA were prepared by fusing splenocytes from BALB/c mice, which had been immunized with poly A . poly U and poly I . poly C, with NS-1 mouse myeloma cells. Of 103 independently derived hybridoma lines which secreted antibodies that reacted with dsRNA, only tw...

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Bibliographic Details
Published in:Phytopathology 1991-02, Vol.81 (2), p.184-187
Main Author: Powell, C.A. (University of Florida, Fort Pierce, FL)
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
ARN
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
PRODUCCION
PRODUCTION
RATON
REACCION ANTIGENO-ANTICUERPO
REACTION ANTIGENE ANTICORPS
SOURIS
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
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Summary:Monoclonal antibodies to double-stranded RNA were prepared by fusing splenocytes from BALB/c mice, which had been immunized with poly A . poly U and poly I . poly C, with NS-1 mouse myeloma cells. Of 103 independently derived hybridoma lines which secreted antibodies that reacted with dsRNA, only two competed favorably with rabbit polyclonal antiserum to poly A . poly U for sites on this synthetic RNA. Further characterization of these two monoclonal antibodies showed that they could detect 3 ng poly A . poly U or 10 ng poly 10 poly C/ml in indirect double antibody sandwich enzyme-linked immunosorbent assay tests. The antibodies did not react with double-stranded DNA, rRNA, or tRNA. They could distinguish between total nucleic extracts from virus-infected and uninfected plants in some but not all cases
ISSN:0031-949X
1943-7684
DOI:10.1094/Phyto-81-184