Loading…
Preparation and characterization of monoclonal antibodies to double-stranded RNA
Monoclonal antibodies to double-stranded RNA were prepared by fusing splenocytes from BALB/c mice, which had been immunized with poly A . poly U and poly I . poly C, with NS-1 mouse myeloma cells. Of 103 independently derived hybridoma lines which secreted antibodies that reacted with dsRNA, only tw...
Saved in:
| Published in: | Phytopathology 1991-02, Vol.81 (2), p.184-187 |
|---|---|
| Main Author: | |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c213t-e84fe532d1c630deb93b0d16f46c0e273667c2477784fe64efea079505a6307c3 |
|---|---|
| cites | |
| container_end_page | 187 |
| container_issue | 2 |
| container_start_page | 184 |
| container_title | Phytopathology |
| container_volume | 81 |
| creator | Powell, C.A. (University of Florida, Fort Pierce, FL) |
| description | Monoclonal antibodies to double-stranded RNA were prepared by fusing splenocytes from BALB/c mice, which had been immunized with poly A . poly U and poly I . poly C, with NS-1 mouse myeloma cells. Of 103 independently derived hybridoma lines which secreted antibodies that reacted with dsRNA, only two competed favorably with rabbit polyclonal antiserum to poly A . poly U for sites on this synthetic RNA. Further characterization of these two monoclonal antibodies showed that they could detect 3 ng poly A . poly U or 10 ng poly 10 poly C/ml in indirect double antibody sandwich enzyme-linked immunosorbent assay tests. The antibodies did not react with double-stranded DNA, rRNA, or tRNA. They could distinguish between total nucleic extracts from virus-infected and uninfected plants in some but not all cases |
| doi_str_mv | 10.1094/Phyto-81-184 |
| format | article |
| fullrecord | <record><control><sourceid>fao_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1094_Phyto_81_184</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US9121684</sourcerecordid><originalsourceid>FETCH-LOGICAL-c213t-e84fe532d1c630deb93b0d16f46c0e273667c2477784fe64efea079505a6307c3</originalsourceid><addsrcrecordid>eNo90EtLAzEQB_AgCtbqzZOnPXg0mtfmcSzFFxQtasFbyOZhV7abkqyH-ulNXfE0zPCbYfgDcI7RNUaK3SzXuyFCiSGW7ABMsGIUCi7ZIZggRDFUTL0fg5OcPxFCQtZ8ApbL5LcmmaGNfWV6V9l16ezgU_s9DmOoNrGPtou96QoZ2ia61udqiJWLX03nYR5SWfWuenmanYKjYLrsz_7qFKzubt_mD3DxfP84ny2gJZgO0EsWfE2Jw5ZT5HyjaIMc5oFxizwRlHNhCRNC7CFnPniDhKpRbYoXlk7B1XjXpphz8kFvU7sxaacx0vs09G8aWmJd0ij8cuRbk63pQnnYtvl_pyZKESQLuxhZMFGbj1TI6lVhgkuK9Ad9Y2lB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Preparation and characterization of monoclonal antibodies to double-stranded RNA</title><source>EZB Electronic Journals Library</source><creator>Powell, C.A. (University of Florida, Fort Pierce, FL)</creator><creatorcontrib>Powell, C.A. (University of Florida, Fort Pierce, FL)</creatorcontrib><description>Monoclonal antibodies to double-stranded RNA were prepared by fusing splenocytes from BALB/c mice, which had been immunized with poly A . poly U and poly I . poly C, with NS-1 mouse myeloma cells. Of 103 independently derived hybridoma lines which secreted antibodies that reacted with dsRNA, only two competed favorably with rabbit polyclonal antiserum to poly A . poly U for sites on this synthetic RNA. Further characterization of these two monoclonal antibodies showed that they could detect 3 ng poly A . poly U or 10 ng poly 10 poly C/ml in indirect double antibody sandwich enzyme-linked immunosorbent assay tests. The antibodies did not react with double-stranded DNA, rRNA, or tRNA. They could distinguish between total nucleic extracts from virus-infected and uninfected plants in some but not all cases</description><identifier>ISSN: 0031-949X</identifier><identifier>EISSN: 1943-7684</identifier><identifier>DOI: 10.1094/Phyto-81-184</identifier><identifier>CODEN: PHYTAJ</identifier><language>eng</language><publisher>St. Paul, MN: American Phytopathological Society</publisher><subject>Agronomy. Soil science and plant productions ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; ARN ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control ; Phytopathology. Animal pests. Plant and forest protection ; Plant viruses and viroids ; PRODUCCION ; PRODUCTION ; RATON ; REACCION ANTIGENO-ANTICUERPO ; REACTION ANTIGENE ANTICORPS ; SOURIS ; VIRUS DE LAS PLANTAS ; VIRUS DES VEGETAUX</subject><ispartof>Phytopathology, 1991-02, Vol.81 (2), p.184-187</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c213t-e84fe532d1c630deb93b0d16f46c0e273667c2477784fe64efea079505a6307c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5299208$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Powell, C.A. (University of Florida, Fort Pierce, FL)</creatorcontrib><title>Preparation and characterization of monoclonal antibodies to double-stranded RNA</title><title>Phytopathology</title><description>Monoclonal antibodies to double-stranded RNA were prepared by fusing splenocytes from BALB/c mice, which had been immunized with poly A . poly U and poly I . poly C, with NS-1 mouse myeloma cells. Of 103 independently derived hybridoma lines which secreted antibodies that reacted with dsRNA, only two competed favorably with rabbit polyclonal antiserum to poly A . poly U for sites on this synthetic RNA. Further characterization of these two monoclonal antibodies showed that they could detect 3 ng poly A . poly U or 10 ng poly 10 poly C/ml in indirect double antibody sandwich enzyme-linked immunosorbent assay tests. The antibodies did not react with double-stranded DNA, rRNA, or tRNA. They could distinguish between total nucleic extracts from virus-infected and uninfected plants in some but not all cases</description><subject>Agronomy. Soil science and plant productions</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>ARN</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant viruses and viroids</subject><subject>PRODUCCION</subject><subject>PRODUCTION</subject><subject>RATON</subject><subject>REACCION ANTIGENO-ANTICUERPO</subject><subject>REACTION ANTIGENE ANTICORPS</subject><subject>SOURIS</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DES VEGETAUX</subject><issn>0031-949X</issn><issn>1943-7684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNo90EtLAzEQB_AgCtbqzZOnPXg0mtfmcSzFFxQtasFbyOZhV7abkqyH-ulNXfE0zPCbYfgDcI7RNUaK3SzXuyFCiSGW7ABMsGIUCi7ZIZggRDFUTL0fg5OcPxFCQtZ8ApbL5LcmmaGNfWV6V9l16ezgU_s9DmOoNrGPtou96QoZ2ia61udqiJWLX03nYR5SWfWuenmanYKjYLrsz_7qFKzubt_mD3DxfP84ny2gJZgO0EsWfE2Jw5ZT5HyjaIMc5oFxizwRlHNhCRNC7CFnPniDhKpRbYoXlk7B1XjXpphz8kFvU7sxaacx0vs09G8aWmJd0ij8cuRbk63pQnnYtvl_pyZKESQLuxhZMFGbj1TI6lVhgkuK9Ad9Y2lB</recordid><startdate>19910201</startdate><enddate>19910201</enddate><creator>Powell, C.A. (University of Florida, Fort Pierce, FL)</creator><general>American Phytopathological Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19910201</creationdate><title>Preparation and characterization of monoclonal antibodies to double-stranded RNA</title><author>Powell, C.A. (University of Florida, Fort Pierce, FL)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c213t-e84fe532d1c630deb93b0d16f46c0e273667c2477784fe64efea079505a6307c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>ANTICORPS MONOCLONAL</topic><topic>ANTICUERPOS MONOCLONALES</topic><topic>ARN</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant viruses and viroids</topic><topic>PRODUCCION</topic><topic>PRODUCTION</topic><topic>RATON</topic><topic>REACCION ANTIGENO-ANTICUERPO</topic><topic>REACTION ANTIGENE ANTICORPS</topic><topic>SOURIS</topic><topic>VIRUS DE LAS PLANTAS</topic><topic>VIRUS DES VEGETAUX</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Powell, C.A. (University of Florida, Fort Pierce, FL)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Powell, C.A. (University of Florida, Fort Pierce, FL)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and characterization of monoclonal antibodies to double-stranded RNA</atitle><jtitle>Phytopathology</jtitle><date>1991-02-01</date><risdate>1991</risdate><volume>81</volume><issue>2</issue><spage>184</spage><epage>187</epage><pages>184-187</pages><issn>0031-949X</issn><eissn>1943-7684</eissn><coden>PHYTAJ</coden><abstract>Monoclonal antibodies to double-stranded RNA were prepared by fusing splenocytes from BALB/c mice, which had been immunized with poly A . poly U and poly I . poly C, with NS-1 mouse myeloma cells. Of 103 independently derived hybridoma lines which secreted antibodies that reacted with dsRNA, only two competed favorably with rabbit polyclonal antiserum to poly A . poly U for sites on this synthetic RNA. Further characterization of these two monoclonal antibodies showed that they could detect 3 ng poly A . poly U or 10 ng poly 10 poly C/ml in indirect double antibody sandwich enzyme-linked immunosorbent assay tests. The antibodies did not react with double-stranded DNA, rRNA, or tRNA. They could distinguish between total nucleic extracts from virus-infected and uninfected plants in some but not all cases</abstract><cop>St. Paul, MN</cop><pub>American Phytopathological Society</pub><doi>10.1094/Phyto-81-184</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-949X |
| ispartof | Phytopathology, 1991-02, Vol.81 (2), p.184-187 |
| issn | 0031-949X 1943-7684 |
| language | eng |
| recordid | cdi_crossref_primary_10_1094_Phyto_81_184 |
| source | EZB Electronic Journals Library |
| subjects | Agronomy. Soil science and plant productions ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES ARN Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control Phytopathology. Animal pests. Plant and forest protection Plant viruses and viroids PRODUCCION PRODUCTION RATON REACCION ANTIGENO-ANTICUERPO REACTION ANTIGENE ANTICORPS SOURIS VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX |
| title | Preparation and characterization of monoclonal antibodies to double-stranded RNA |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T21%3A59%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-fao_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20and%20characterization%20of%20monoclonal%20antibodies%20to%20double-stranded%20RNA&rft.jtitle=Phytopathology&rft.au=Powell,%20C.A.%20(University%20of%20Florida,%20Fort%20Pierce,%20FL)&rft.date=1991-02-01&rft.volume=81&rft.issue=2&rft.spage=184&rft.epage=187&rft.pages=184-187&rft.issn=0031-949X&rft.eissn=1943-7684&rft.coden=PHYTAJ&rft_id=info:doi/10.1094/Phyto-81-184&rft_dat=%3Cfao_cross%3EUS9121684%3C/fao_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c213t-e84fe532d1c630deb93b0d16f46c0e273667c2477784fe64efea079505a6307c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |