Differential protection against papaya ringspot virus isolates in coat protein gene transgenic papaya and classically cross-protected papaya

Transgenic papaya expressing the coat protein gene of the mild papaya ringspot virus strain from Hawaii (PRV HA 5-1) showed high levels of resistance against the severe PRV HA isolate from Hawaii. Inoculation with high concentrations of the virus, multiple mechanical inoculations or graft inoculatio...

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Bibliographic Details
Published in:Phytopathology 1994-11, Vol.84 (11), p.1359-1366
Main Authors: Tennant, P.F. (Cornell University, Geneva, NY.), Gonsalves, C, Ling, K.S, Fitch, M, Manshardt, R, Slightom, J.L, Gonsalves, D
Format: Article
Language:English
Subjects:
AMELIORATION DES PLANTES
ANATOMIA DE LA PLANTA
ANATOMIE VEGETALE
Biological and medical sciences
Biotechnology
CARICA PAPAYA
FITOMEJORAMIENTO
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
HAWAI
HAWAII
PATHOTYPE
PATOTIPOS
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
PODER PATOGENO
POTEXVIRUS
POUVOIR PATHOGENE
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
SINTOMAS
SYMPTOME
VARIACION GENETICA
VARIATION GENETIQUE
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
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Summary:Transgenic papaya expressing the coat protein gene of the mild papaya ringspot virus strain from Hawaii (PRV HA 5-1) showed high levels of resistance against the severe PRV HA isolate from Hawaii. Inoculation with high concentrations of the virus, multiple mechanical inoculations or graft inoculations failed to break the resistance of transgenic papaya. Virus recovery assays from these inoculated plants suggested that virus replication and movement were impaired. Transgenic papaya also showed high levels of resistance against severe PRV isolates recently collected from Hawaii. Similarly, PRV HA 5-1 cross-protected papaya offered high levels of protection against two of the three isolates from Hawaii. However, neither transgenic nor mild strain-infected papaya showed good levels of protection against PRV isolates from 11 other geographical regions that were serologically related to PRV HA 5-1. A range of reactions was observed: complete resistance; delay in symptom development and symptom attenuation with PRV isolates from the Bahamas, Florida, and Mexico; and, a shorter delay in symptom development but no symptom attenuation with isolates from Brazil or Thailand
ISSN:0031-949X
1943-7684
DOI:10.1094/Phyto-84-1359