Identification, partial sequencing, and detection of a potyvirus from Kalanchoe blossfeldiana

• Published in: Phytopathology 1994, Vol.84 (2), p.161-166
• Main Authors: HUSTED, K, BECH, K, ALBRECHTSEN, M, BORKHARDT, B
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control; isolation techniques; kalanchoe blossfeldiana; nucleotide sequence; pathogenicity; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; poder patogeno; potyvirus; potyviruses; pouvoir pathogene; purificacion; purification; secuencia nucleica; sequence nucleique; sintomas; symptome; symptoms; technique de l' isolement; tecnicas de aislamiento
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/Phyto-84-161

Flexuous, filamentous virus particles with an average length of 724 nm and showing a characteristic green island mosaic pattern were observed in Kalanchoe blossfeldiana. The virus was sap inoculated from naturally infected K. blossfeldiana plants to Chenopodium quinoa and purified from these plants. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the capsid protein size was estimated to be 33 kDa. Of 14 plant species inoculated, only C. quinoa and C. amaranticolor showed local symptoms. Immunosorbent electron microscopy showed the virus to be serologically unrelated to 10 other potyviruses. The virus was aphid transmitted. This ability was lost, however, after serial mechanical transmissions. The intact virions were used for antiserum preparation, and RNA was purified from virions for cDNA cloning and sequencing. A database search showed the virus to be closely related to but distinct from turnip mosaic potyvirus. On the basis of these investigations, it is concluded that the virus is a hitherto undescribed potyvirus, and the name Kalanchoe mosaic potyvirus is suggested. The prepared antiserum was very specific and sensitive in enzyme-linked immunosorbent assays, and this method is recommended for routine testing.