Mycelial compatibility groups in Texas peanut field populations of Sclerotium rolfsii

Sclerotium rolfsii isolates (366) collected from 1985 to 1994 in Texas peanut fields were placed in 25 mycelial compatibility groups (MCG) based on the formation of an antagonism zone between incompatible mycelia of paired isolates. The same MCG were often detected in several geographically distant...

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Bibliographic Details
Published in:Phytopathology 1995, Vol.85 (12), p.1507-1512
Main Authors: Nalim, F.A. (Texas AandM University, College Station.), Starr, J.L, Woodard, K.E, Segner, S, Keller, N.P
Format: Article
Language:English
Subjects:
ADN
AGENT PATHOGENE
ARACHIS HYPOGAEA
Biological and medical sciences
CORTICIUM ROLFSII
ENFERMEDADES FUNGOSAS
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
GENETICA
GENETIQUE
MALADIE FONGIQUE
MICELIO
MYCELIUM
ORGANISMOS PATOGENOS
PATHOTYPE
PATOTIPOS
Phytopathology. Animal pests. Plant and forest protection
TEXAS
VARIACION GENETICA
VARIATION GENETIQUE
Variation, races, biotypes, parasitic specialization, genetics
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Summary:Sclerotium rolfsii isolates (366) collected from 1985 to 1994 in Texas peanut fields were placed in 25 mycelial compatibility groups (MCG) based on the formation of an antagonism zone between incompatible mycelia of paired isolates. The same MCG were often detected in several geographically distant counties in Texas. Individual peanut contained one to five MCG, whereas individual plants were infected with isolates from one MCG. Two fields sampled extensively in 1992 maintained the same MCG in 1993: MCG 6 and 7 in Galen and MCG 1, 2, 3, 4, and 12 in Grissom. However, MCG 11, which consisted of a single fungicide-tolerant 1985 isolate of S. rolfsii from Galen, was not found among the 366 isolates collected nor was the trait of fungicide tolerance. DNA amplification patterns resulting from the use of the 18-base oligonucleotide primer NK2 and from restriction digests of the internal transcribed spacer (ITS) region of the rDNA were examined in a subset of 80 isolates from four fields to determine the genetic similarity of isolates within and between 12 MCG. Three NK2-amplified DNA patterns in genomic DNA and four MboI restriction digest patterns of the ITS region were found. All isolates within a MCG gave identical patterns for each marker, and some MCG shared the same ITS and NK2 patterns. MCG sharing the same DNA patterns were often from the same field
ISSN:0031-949X
1943-7684
DOI:10.1094/phyto-85-1507