A novel method for tospovirus acquisition by thrips

An in vitro feeding method for the acquisition of impatiens necrotic spot tospovirus (INSV) and tomato spotted wilt tospovirus (TSWV) by western flower thrips (Frankliniella occidentalis (Pergande) was developed. This is the first system developed in which tospovirus viability was maintained in an i...

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Bibliographic Details
Published in:Phytopathology 1995-04, Vol.85 (4), p.480-483
Main Authors: Hunter, W.B. (USDA, ARS, U.S. National Arboretum, Beltsville, MD.), Hsu, H.T, Lawson, R.H
Format: Article
Language:English
Subjects:
Biological and medical sciences
CALIFORNIA
CALIFORNIE
DATURA STRAMONIUM
ELISA
FRANKLINIELLA OCCIDENTALIS
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
HAWAI
HAWAII
HOTE ALTERNATIF
HUESPEDES ALTERNATIVOS
LACTUCA SATIVA
LARVAS
LARVE
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
PODER PATOGENO
POUVOIR PATHOGENE
SOLANACEAE
TEST ELISA
TRANSMISION DE ENFERMEDADES
TRANSMISSION DES MALADIES
VECTEUR DE MALADIE
VECTORES
VIABILIDAD
VIABILITE
VIRUS DE LAS PLANTAS
VIRUS DEL BRONCEADO DEL TOMATE
VIRUS DES VEGETAUX
VIRUS MALADIE BRONZEE TOMATE
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Description
Summary:An in vitro feeding method for the acquisition of impatiens necrotic spot tospovirus (INSV) and tomato spotted wilt tospovirus (TSWV) by western flower thrips (Frankliniella occidentalis (Pergande) was developed. This is the first system developed in which tospovirus viability was maintained in an in vitro technique specifically designed for thrips feeding. Three-day-old larvae of western flower thrips were caged in a cylindrical plastic tube with Parafilm membrane covering both ends. Leaf extracts from INSV- and TSWV-infected Nicotiana benthamiana or Datura stramonium were prepared. Following one cycle of differential centrifugation, the extract was placed on the membrane on one end of the cage. A second membrane was placed over the first membrane thus covering the extract. After a 24-h acquisition access feeding period, thrips were kept on green-bean pods in a container until they were 10-day-old adults. Enzyme-linked immunosorbent assays (ELISA) showed that acquisition feeding of virus suspensions prepared from infected plant hosts resulted in thrips testing positive for viruses. Cohorts from these ELISA-positive thrips were shown to be viruliferous when placed onto virus-susceptible host plants. This in vitro method is an important tool in the study of tospovirus/thrips interactions
ISSN:0031-949X
1943-7684
DOI:10.1094/Phyto-85-480