In situ localization of barley yellow dwarf virus coat protein in oats

The expression of barley yellow dwarf virus (BYDV) coat protein was followed in situ by immunogold labeling of ultrathin sections. Samples of BYDV-PAV-IL-infected oat seedlings were taken 1-10 days postinoculation (dpi) and prepared for electron microscopy. Gold labeling was first detected in the ho...

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Bibliographic Details
Published in:Phytopathology 1995, Vol.85 (5), p.556-560
Main Authors: NASS, P. H, JAKSTYS, B. P, D'ARCY, C. J
Format: Article
Language:English
Subjects:
avena byzantina
barley yellow dwarf luteovirus
Biological and medical sciences
biological differences
cell structure
chemistry
chimie
developmental stages
diferencias biologicas
difference biologique
estructura celular
etapas de desarrollo
floema
Fundamental and applied biological sciences. Psychology
immunologie
immunology
inmunologia
luteovirus enanismo amarillo cebada
luteovirus jaunisse nanisante orge
pathogenicity
phloem
phloeme
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
poder patogeno
pouvoir pathogene
proteinas
proteine
proteins
quimica
rhopalosiphum padi
stade de developpement
structure cellulaire
Systematics. Structure, properties and multiplication. Genetics
ultraestructura
ultrastructure
vecteur de maladie
vectores
vectors
xilema
xylem
xyleme
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Summary:The expression of barley yellow dwarf virus (BYDV) coat protein was followed in situ by immunogold labeling of ultrathin sections. Samples of BYDV-PAV-IL-infected oat seedlings were taken 1-10 days postinoculation (dpi) and prepared for electron microscopy. Gold labeling was first detected in the host cytoplasm at 2 dpi. At 3 dpi, labeling had increased and was found mostly around or within regions that contained ribosome associated filamentous material. No labeling was found over densely compressed filaments. By 4-5 dpi, viral coat protein also was detected in the nucleus. Viral coat protein was not associated with any other cellular components. The results suggest that viral coat protein is first expressed in the cytoplasm of the infected host cell. Since labeling was mostly found in or around regions of ribosome-associated filaments, it is proposed that coat protein is expressed by cytoplasmic ribosomes from viral RNA. Coat protein found in the nucleus during later stages of infection probably diffused into the nucleoplasm after disruption of the nuclear membrane.
ISSN:0031-949X
1943-7684
DOI:10.1094/Phyto-85-556