Effect of Stem Cell Activation, Culture Media of Manipulated Embryos, and Site of Embryo Transfer in the Production of F0 Embryonic Stem Cell Mice1

• Published in: Biology of reproduction 2009-06, Vol.80 (6), p.1216-1222
• Main Authors: Ramírez, Miguel Angel, Fernández-González, Raúl, Pérez-Crespo, Miriam, Pericuesta, Eva, Gutiérrez-Adán, Alfonso
• Format: Article
• Language: English
• Subjects: cell activation; culture media; embryo transfer; embryonic stem cell; mouse
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod.108.075044

Recently, F0 embryonic stem (ES) cell mice have been produced by injection of ES cells into eight-cell embryos using either laser- or piezo-assisted injection systems. To simplify the injection procedure, we have optimized the conventional blastocyst injection method, free of laser- or piezo-assisted micromanipulation systems, to produce F0 ES cell pups. To increase the efficiency of producing mice from ES cell injection into eight-cell and blastocyst stage embryos, we have tested: 1) the effect of activating ES cell before injection, 2) the effect of in vitro culture in medium optimized for the survival of both ES cells and embryos, and 3) the effect of transferring the micromanipulated embryos into the oviduct versus into the uterus of CD1 foster mice. Two B6D2 hybrid ES cell lines were used for injection in a multifactorial analysis to evaluate the efficiency of producing live chimeric and F0 ES cell mice. Our results demonstrate that the activation of ES cells and the appropriate culture conditions are crucial parameters influencing the generation of F0 ES cell offspring. Transfer of blastocysts injected with ES cells into the oviduct of 0.5-day postcoitum pseudopregnant females increased the number of live animals with higher chimera proportion. Under these conditions, injections into eight-cell embryos produce a high number of F0 ES mice, and the conventional blastocyst injection method produces a lower number of F0 ES cell pups; however, the efficiency of production of chimeric mice with germline transmission was high. We have developed an economical and efficient technique for producing fully ES cell-derived F0 mice with full germline transmission that can be applied in many laboratories without the use of piezo or laser instruments.