The role of physical exercise in apoptosis in an experimental cerebral ischemia model

• Published in: The FASEB journal 2022-05, Vol.36 (S1), p.n/a
• Main Authors: Souza, Gabriel P. N., Porsani, Lucas B., Tirapelli, Victor C., Stevanato, Ana Laura R., Novais, Paulo C., Assis Cirino, Mucio L., Tirapelli, Daniela, Tirapelli, Luis F.
• Format: Article
• Language: English
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fasebj.2022.36.S1.L8057

Introduction Stroke is a major public health problem, with high mortality rates and high levels of disability. Between 1990 and 2010 for example, stroke increased from the fifth to the third leading cause of disability. In addition, its incidence has increased among younger people, a situation in which there are significant health consequences and elevated social costs. There is evidence that physical exercise promotes neuroprotective effects when used as a therapeutic treatment in an experimental ischemia model. However, the mechanisms of neuroprotection are still not well understood. Objective The objective of this study was to evaluate the expression profile of the microRNAs miR‐21, miR‐16, miR‐155 and caspase‐3 and Bcl‐2 genes by real‐time PCR in brain tissue (ischemic focus) of the animals after the performance of physical training before focal cerebral ischemia. Materials and Methods 48 Wistar rats were used. The animals were divided into four experimental groups: control group, ischemia group, exercise group and exercise + ischemia group. The real‐time PCR methodology was used to analyze the expression of miRNAs; miR16, miR‐21, miR‐155, caspase‐3 and Bcl‐2. Results and conclusions In our study, we did not observe statistically significant differences in the expression of miRNAs: miR‐16, miR‐21, miR‐155 or in the Bcl‐2 and caspase‐3 genes in brain tissue in the groups undergoing cerebral ischemia and exercise when compared to the control group.