Direct Effects of VEGF and VEGF Receptor Inhibitor on Proliferation of Human Renal Proximal Tubular Epithelial Cells (HRPTEC)

Clinical models link VEGF inhibition to hypertension. However, the role of VEGF in renal mechanisms of hypertension is poorly understood. We seek to determine if VEGF or VEGF receptor inhibitor (SU5416) directly affects proliferation of cultured HRPTEC, compared to human umbilical vein endothelial c...

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Bibliographic Details
Published in:The FASEB journal 2006-03, Vol.20 (4), p.A764-A764
Main Authors: Gu, Jian‐Wei, Shparago, Megan, Tan, Wei, Bailey, Amelia Purser
Format: Article
Language:English
Online Access:Get full text
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Summary:Clinical models link VEGF inhibition to hypertension. However, the role of VEGF in renal mechanisms of hypertension is poorly understood. We seek to determine if VEGF or VEGF receptor inhibitor (SU5416) directly affects proliferation of cultured HRPTEC, compared to human umbilical vein endothelial cells (HUVEC), and human aortic smooth muscle cells (HASMC). 3H‐thymidine incorporation was determined in those cells following exposure to VEGF (10 ng/ml), VEGF plus SU5416 (10 μmol/L), SU5416 (1, 5, 10, & 20 μmol/L), vehicle control for 18 hrs. VEGF slightly but significantly increased proliferation of HRPTEC, compared to the control (10%, P=0.012, n=6). There was a significant decrease in proliferation of HRPTEC treated with VEGF plus SU5416, compared to the control (65%, P
ISSN:0892-6638
1530-6860
DOI:10.1096/fasebj.20.4.A764-a