Tissue specific expression of transcripts from multiple Sry loci in male rats

We have identified multiple Sry loci on the rat Y chromosome. In this study, we asked whether all Sry copies are expressed in adrenal gland, kidney and testis, and if each locus is expressed at the same level in each tissue. RNA from five 15 week‐old male rats was reverse transcribed, and cDNAs ampl...

Full description

Saved in:
Bibliographic Details
Published in:The FASEB journal 2007-04, Vol.21 (5), p.A476-A476
Main Authors: Dunmire, Jeffrey, Farkas, Joel, Ely, Daniel, Turner, Monte, Milsted, Amy
Format: Article
Language:English
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have identified multiple Sry loci on the rat Y chromosome. In this study, we asked whether all Sry copies are expressed in adrenal gland, kidney and testis, and if each locus is expressed at the same level in each tissue. RNA from five 15 week‐old male rats was reverse transcribed, and cDNAs amplified using primer sets that produce amplicons of varying lengths that correspond to specific Sry copies. Fluorescent labeled PCR products were detected using the ABI 3130xl Genetic Analyzer and analyzed using GeneMapper v4.0 software (Applied Biosystems). Transcript amount from a particular Sry locus was calculated as a percentage of total Sry transcript present in a given sample. Results show that transcripts from each Sry locus are present in all three tissues and the relative amount of each copy expressed differs among tissues. We report significant differences in Sry2 and Sry3C expression in adrenal gland and kidney compared to testis (P=
ISSN:0892-6638
1530-6860
DOI:10.1096/fasebj.21.5.A476-c