Anti‐inflammatory Effects of Conjugated Linoleic Acids: Distinct isomer‐specific effects on gene expression in mouse macrophage cells

• Published in: The FASEB journal 2007, Vol.21 (6), p.A736-A736
• Main Authors: Lee, Yunkyoung, Heuvel, Jack Vanden
• Format: Article
• Language: English
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fasebj.21.6.A736-c

Conjugated linoleic acid (CLA) has anti‐inflammatory effects in vitro and in vivo; however the contribution of specific isomers to this effect has not been extensively studied. RAW264.7 (RAW) cells, mouse macrophage cells, were treated with five different CLA isomers (9E11E, 9Z11E, 9Z11Z, 10E12Z, and 11Z13E) and gene expression microarray was performed and significantly regulated genes by CLA isomers were collected and verified by a real‐time PCR. Examination of the biological functions of regulated genes by the five CLA isomers showed distinct properties. Interestngly, 9E11E regulates the largest number of genes in RAW cells and it was the only isomer to increase interleukin 1 receptor antagonist (IL‐1Ra). Using PPAR and RXR specific antagonists and coactivator recruitment assays, it was evident that multiple mechanisms were responsible for gene regulation by CLA isomers. 9E11E increased IL‐1Ra but decreased of IL‐1α, IL‐1β and IL‐6 at both the mRNA and protein level. The increase of IL‐1Ra by 9E11E was transcriptionally regulated and was abolished by an inhibitor of PI3K. In conclusion, despite structurally similarity the five CLA isomers show specificity in the alteration of various genes in mouse macrophage cells. The regulation of anti‐ and pro‐inflammatory cytokines by 9E11E in RAW cells demonstrates a potential new therapeutic use of this fatty acids.