Activation of toll‐like receptors 2 or 4 impairs hypoxic pulmonary vasoconstriction

Toll like receptors (TLRs) play a pivotal role in sepsis, but their contribution to sepsis‐induced impairment of HPV is unknown. To assess the impact of TLRs on HPV, left lung pulmonary vascular resistance (LPVR) was measured before and during left mainstem bronchus occlusion (LMBO) in wild‐type (WT...

Full description

Saved in:
Bibliographic Details
Published in:The FASEB journal 2007, Vol.21 (6), p.LB113-LB113
Main Authors: Petersen, Bodil, Ichinose, Fumito, Shin, Hae‐Sook, Shigematsu, Misako, Bagchi, Aranya, Bloch, Kenneth D, Zapol, Warren M, Hellman, Judith
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Toll like receptors (TLRs) play a pivotal role in sepsis, but their contribution to sepsis‐induced impairment of HPV is unknown. To assess the impact of TLRs on HPV, left lung pulmonary vascular resistance (LPVR) was measured before and during left mainstem bronchus occlusion (LMBO) in wild‐type (WT), TLR2−/−, and TLR4−/− mice. We studied HPV 22 hours after IV challenge with saline, or a TLR2 agonist (Pam3Cys, 20mg/kg), or a TLR4 agonist (lipopolysaccharide, LPS, 20mg/kg). In additional mice, PMN were depleted prior to the challenge with saline, Pam3Cys or LPS. Furthermore, circulating white blood cell (WBC) concentration and lung myeloperoxidase (MPO) activity were measured 22 hours after challenge with saline, Pam3Cys or LPS. In saline‐challenged WT mice, LMBO doubled LPVR, while the ability of LMBO to increase LPVR was impaired in Pam3Cys‐ or LPS‐challenged mice (P
ISSN:0892-6638
1530-6860
DOI:10.1096/fasebj.21.6.LB113