VACM‐1, a cul 5 Gene, regulates Aquaporin‐1 Levels in vitro

VACM‐1/cul 5 cDNA expression in COS‐1 cells inhibits cell growth. The mechanism of this regulation involves MAPK phosphorylation, but the direct target of VACM‐1 activity is not known. To identify genes that may be comprimised by overexpression of VACM‐1 cDNA, we performed microarray analysis. Our r...

Full description

Saved in:
Bibliographic Details
Published in:The FASEB journal 2008-04, Vol.22 (S2), p.226-226
Main Authors: Stodola, Joseph L., Burnatowska‐Hledin, Maria
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:VACM‐1/cul 5 cDNA expression in COS‐1 cells inhibits cell growth. The mechanism of this regulation involves MAPK phosphorylation, but the direct target of VACM‐1 activity is not known. To identify genes that may be comprimised by overexpression of VACM‐1 cDNA, we performed microarray analysis. Our results showed that in COS‐1 cells transfected with VACM‐1 cDNA, mRNA levels for several genes including Aquaporin‐1 (AQP1) were decreased. To extend these observations further we examined the effects of VACM‐1 on AQP1 in COS‐1 cells at the protein level. Our results indicate that in COS‐1 cells transfected with VACM‐1 cDNA, endogenous expression of AQP1 protein is decreased significantly (n=6, p
ISSN:0892-6638
1530-6860
DOI:10.1096/fasebj.22.2_supplement.226