Protein Dynamics across splanchnic and skeletal muscle beds following co‐ingestion of whey protein and casein in humans

• Published in: The FASEB journal 2008-04, Vol.22 (S2), p.693-693
• Main Authors: Soop, Mattias, Nehra, Vandana, Bigelow, Maureen L, Boirie, Yves, Ford, G. Charles, Nair, K. Sreekumaran
• Format: Article
• Language: English
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fasebj.22.2_supplement.693

It has been shown ingestion of whey protein (WP) results in rapid systemic appearance rates of amino acids (AA) but in higher amino acid (AA) oxidation and whole body protein synthesis than in with casein (cas) ingestion which inhibits protein breakdown. To determine whether combined ingestion of both cas and WP in a meal containing carbohydrate has any disparate effects on protein economy in splanchnic and muscle beds we studied 12 healthy volunteers who ingested intrinsically labeled WP and Cas protein fractions. Following ingestion of 0.625 g·kg FFM−1 each of [15N]WP and [13C]Cas and 0.9 g·kg FFM−1 of lactose; or 0.625 g·kg FFM−1 each of [13C]WP and [15N]CAS and 0.9 g·kg FFM−1 of lactose blood samples were collected from femoral artery and vein and hepatic vein at frequent intervals. At 80–120 min, the rate of appearance (PheRa) of Phe at hepatic vein from WP and Cas were similar (5.5 (0.9) vs 5.2 (0.7) μmol, min−1, but at 220–260 min, PheRa from WP was slower than PheRa from Cas (2.4 (0.5) vs 5.3 (1.2) μmol, min−1, P