Identification of Peptides Derived from Azuki Bean (Vigna angularis) which Stimulate Cholecystokinin Secretion from the Enteroendocrine STC‐1 Cells

• Published in: The FASEB journal 2010-04, Vol.24 (S1), p.lb265-lb265
• Main Authors: Sufian, Md. Kaosar Niaz Bin, Hira, Tohru, Hara, Hiroshi
• Format: Article
• Language: English
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fasebj.24.1_supplement.lb265

Objectives We found appetite‐suppressive peptides through cholecystokinin (CCK) secretion in vivo from a peptic digest of soybean β‐conglycinin. Later we found that peptic digest of dolicholin, a newly identified protein in country beans (Dolichos lablab), similar to β‐conglycinin, more potently stimulate CCK secretion from enteroendocrine cells. Our present study aimed to screen new CCK‐releasing peptides from beans that exist with homologous protein to dolicholin and to identify the active peptides therein. Methods Five kinds of beans were hydrolyzed with pepsin and were examined for CCK‐releasing activities in the enteroendocrine cell line STC‐1. Results Peptones prepared from azuki bean (ABP), mung bean (Vigna radiata) and country bean containing closely homologous protein were potent to stimulate CCK secretion in the STC‐1 cells, with the highest activity by ABP. Acetonitrile soluble peptides in ABP stimulated CCK secretion, and pronase treatment abolished its activity. Average molecular size of ABP was estimated to be ~3 kDa, and fractions containing 3–5 kDa peptides showed highest CCK‐releasing activities. Conclusions These results indicate that peptides in ABP are potent to stimulate CCK secretion and also suggest that the CCK‐releasing peptides are derived from protein similar to dolicholin. (This research was supported by Japan Society for the Promotion of Science).