AcSDKP Reduces Collagen Crosslinking, MMP and NFKB Expression in Angiotensin II Induced Hypertension

• Published in: The FASEB journal 2010-04, Vol.24 (S1), p.lb572-lb572
• Main Authors: Gonzalez, German Esteban, Nakagawa, Pablo, Liu, Yunhe, Liao, Tang‐Dong, Yang, Xiao‐Ping, Rhaleb, Nour‐Eddine, Carretero, Oscar A.
• Format: Article
• Language: English
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fasebj.24.1_supplement.lb572

We hypothesized that N‐acetyl‐Ser‐Asp‐Lys‐Pro AcSDKP not only reduces fibrosis but also crosslinking. Moreover we tested whether it increases MMP, which has antifibrotic effects and whether it reduces NFκB in the heart. Lewis rats were divided in 4 groups (n=6, each): 1 (control); 2 (AngII 750…g/kg/day); 3 (AcSDKP 800…g/kg/day); and 4 (AngII+AcSDKP). Systolic blood pressure (mmHg) was recorded for 3 weeks. After 3 weeks crosslinking was quantified by measuring total (TC), insoluble (IC) and soluble (SC) collagen (μg/mg dry weight tissue) and MMP2 activity (% respect to 1) in cardiac tissue. NFκB activity was quantified (EMSA assay). Results (X±SEM) Control AngII AcSDKP AcSDKP+AngII BP 125±3 202±15* 122±3 205±8* TC 11.7±1.3 19.0±1.8* 12.5±1.5 13.0±0.9# IC 9.5±1.3 16.3±1.6* 10.7±1.3 10.0±0.9# SC 2.0±0.2 2.7±0.7 1.7±0.4 2.9±0.7 MMP2 100.0±19.2 101.9±13.0 74.9±13.1 139.6±19.5† NFκB 11.5±1.4 24.4±1.8* 15.0±3.5 15.7±2.0# * and #p