Synthesis and Functional Analysis of a Structure‐Switching Aptamer with Homogeneous 3′‐ends

A structure‐switching signaling aptamer that could detect the antibiotic tobramycin was previously produced by a novel in vitro selection strategy. To facilitate the analysis of the mechanism by which this RNA detects tobramycin, the aptamer was synthesized in a manner that ensures that the 3′ ends...

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Bibliographic Details
Published in:The FASEB journal 2011-04, Vol.25 (S1), p.lb47-lb47
Main Authors: Baltis, Ariel, Morse, Daniel Paul
Format: Article
Language:English
Online Access:Get full text
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Summary:A structure‐switching signaling aptamer that could detect the antibiotic tobramycin was previously produced by a novel in vitro selection strategy. To facilitate the analysis of the mechanism by which this RNA detects tobramycin, the aptamer was synthesized in a manner that ensures that the 3′ ends of the RNA are homogeneous. This was done by incorporating a self‐cleaving ribozyme at the 3′ end. The resulting RNA will be subjected to enzyme and lead ion‐catalyzed hydrolysis to confirm that the ends are homogenous and to analyze the nature of the tobramycin‐induced conformational change.
ISSN:0892-6638
1530-6860
DOI:10.1096/fasebj.25.1_supplement.lb47